碱性蛋白酶对黑麦草叶蛋白水解作用的研究

hrough single factors and L9 (34) Orthogonal experiment, the limited process of hydrolysis for ryegrass leaf protein concentration (RLPC) with alcalase was studied systematically. The optimum conditions were established, which including hydrolyzing temperature (45℃), the proportions of E/S (1750 units per gram), pH (10.5) and reaction time (10 hours). The profiles of High Performance Liquid Chromatography (HPLC) showed that there were 7 kinds of peptides in the protein hydrolysates under the best hydrolysis conditions, their relative molecular weights were between 102u and 14502u, the content of peptides which average molecular weight was 249u had the highest propotion which was 21.13%. The profiles of Automatic Amino Acid Analyzer (AAAA) showed that the total content of free amino acid in hydrolysates was 3.233%, the proportion of essential amino acid in total amino acid was 30.776%, the proportion of sapor amino acid was 38.324%, and the proportion of drug-effective amino acid was 75.286%. In addition, the results indicated that the concentration of hydrolyzed ryegrass leaf protein could be used as an additive in food industry potentially.