绿原酸的微波超声波联合提取及其抑菌作用

he purpose of this passage is to find a new and better method to extract chlorogenic acid from honeysuckle-flowers and its bacteticidal effect Microwave extraction, ultrasonic extraction and co-microwave ultrasonic extraction were studied, the result showed that co-microwave ultrasonic extraction was the best method. Optimal conditions for the extraction of cholorogenic acid were obtained: the chlorogenic acid extracted by 70% ethanol with the ratio(material to solvent) of 1∶20, ultrasonic 18 W, ultrasonic extract 20 min, the extraction rate of cholorogenic acid could be more than 73% in these conditions. The bacteticidal active test of chlorogenic acid showed that chlorogenic acid had more effective to Gram positive bacteria than Gram negative bacteria and had the same effect to Gram negative bacteria compared with Kanamycin (0.1 mg/ml),the minimum inhibitory concentration of cholorogenic acid to shigella or salmonella was 0.125 mg/ml.