SARS-CoV-2 infection, disease and transmission in domestic cats
SARS-CoV-2 infection, disease and transmission in domestic cats
Abstract Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) is the cause of Coronavirus Disease 2019 (COVID-19) and responsible for the current pandemic. Recent SARS-CoV-2 susceptibility and transmission studies in cats show that the virus can replicate in these companion animals and transmit to other cats. Here, we present an in-depth study of SARS-CoV-2 infection, associated disease and transmission dynamics in domestic cats. Six 4- to 5-month-old cats were challenged with SARS-CoV-2 via intranasal and oral routes simultaneously. One day post challenge (DPC), two sentinel contact cats were co-mingled with the principal infected animals. Animals were monitored for clinical signs, clinicopathological abnormalities and viral shedding throughout the 21 DPC observation period. Postmortem examinations were performed at 4, 7 and 21 DPC to investigate disease progression. Viral RNA was not detected in blood but transiently in nasal, oropharyngeal and rectal swabs and bronchoalveolar lavage fluid as well as various tissues. Tracheobronchoadenitis of submucosal glands with the presence of viral RNA and antigen was observed in airways of the infected cats on 4 and 7 DPC. Serology showed that both, principal and sentinel cats, developed SARS-CoV-2-specific and neutralizing antibodies to SARS-CoV-2 detectable at 7 DPC or 10 DPC, respectively. All animals were clinically asymptomatic during the course of the study and capable of transmitting SARS-CoV-2 to sentinels within 2 days of comingling. The results of this study are critical for our understanding of the clinical course of SARS-CoV-2 in a naturally susceptible host species, and for risk assessment of the maintenance of SARS-CoV-2 in felines and transmission to other animals and humans.
Gaudreault Natasha N.、Richt Juergen A.、Trujillo Jessie D.、Carossino Mariano、Madden Daniel W.、Indran Sabarish V.、Balaraman Velmurugan、Kwon Taeyong、Artiaga Bianca Libanori、Morozov Igor、Garc¨aa-Sastre Adolfo、Bold Dashzeveg、Balasuriya Udeni B. R.、Meekins David A.、Cool Konner、Henningson Jamie、Ma Wenjun、Wilson William C.
Department of Diagnostic Medicine/Pathobiology, College of Veterinary Medicine, Kansas State UniversityDepartment of Diagnostic Medicine/Pathobiology, College of Veterinary Medicine, Kansas State UniversityDepartment of Diagnostic Medicine/Pathobiology, College of Veterinary Medicine, Kansas State UniversityLouisiana Animal Disease Diagnostic Laboratory and Department of Pathobiological Sciences, School of Veterinary Medicine, Louisiana State UniversityDepartment of Diagnostic Medicine/Pathobiology, College of Veterinary Medicine, Kansas State UniversityDepartment of Diagnostic Medicine/Pathobiology, College of Veterinary Medicine, Kansas State UniversityDepartment of Diagnostic Medicine/Pathobiology, College of Veterinary Medicine, Kansas State UniversityDepartment of Diagnostic Medicine/Pathobiology, College of Veterinary Medicine, Kansas State UniversityDepartment of Diagnostic Medicine/Pathobiology, College of Veterinary Medicine, Kansas State UniversityDepartment of Diagnostic Medicine/Pathobiology, College of Veterinary Medicine, Kansas State UniversityDepartment of Microbiology, Icahn School of Medicine at Mount Sinai, New York||Global Health and Emerging Pathogens Institute, Icahn School of Medicine at Mount Sinai, New York||Department of Medicine, Division of Infectious Diseases, Icahn School of Medicine at Mount Sinai, New York||The Tisch Cancer Institute, Icahn School of Medicine at Mount SinaiDepartment of Diagnostic Medicine/Pathobiology, College of Veterinary Medicine, Kansas State UniversityLouisiana Animal Disease Diagnostic Laboratory and Department of Pathobiological Sciences, School of Veterinary Medicine, Louisiana State UniversityDepartment of Diagnostic Medicine/Pathobiology, College of Veterinary Medicine, Kansas State UniversityDepartment of Diagnostic Medicine/Pathobiology, College of Veterinary Medicine, Kansas State UniversityDepartment of Diagnostic Medicine/Pathobiology, College of Veterinary Medicine, Kansas State UniversityDepartment of Diagnostic Medicine/Pathobiology, College of Veterinary Medicine, Kansas State UniversityArthropod Borne Animal Disease Research Unit, Agricultural Research Service, United States Department of Agriculture
医学研究方法基础医学微生物学
SARS-CoV-2COVID-19felinescatspetstransmission
Gaudreault Natasha N.,Richt Juergen A.,Trujillo Jessie D.,Carossino Mariano,Madden Daniel W.,Indran Sabarish V.,Balaraman Velmurugan,Kwon Taeyong,Artiaga Bianca Libanori,Morozov Igor,Garc¨aa-Sastre Adolfo,Bold Dashzeveg,Balasuriya Udeni B. R.,Meekins David A.,Cool Konner,Henningson Jamie,Ma Wenjun,Wilson William C..SARS-CoV-2 infection, disease and transmission in domestic cats[EB/OL].(2025-03-28)[2025-07-16].https://www.biorxiv.org/content/10.1101/2020.08.04.235002.点此复制
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