家蚕F-box only蛋白BmFBXO21的抗体制备和定位分析

he eukaryotic F-box protein family is characterized by an F-box motif that has been shown to be critical for the controlled degradation of regulatory proteins. We identified a gene encoding an F-box only protein from a cDNA library of silkworm pupae, whose GenBank Accession Number is DN237283.1, and we named it BmFBXO21. Bioinformatic analysis on the amino acid sequence shows that BmFBXO21 has a low degree of similarity to proteins from other species, and may be related to the regulation of cell-cycle progression. After we had prepared the polyclonal antibodies, we used ELISA to detect the titer, which was larger than 1:12800, and the specific reaction responsed very well in Western blot analysis. We have detected the expression pattern of BmFBXO21 mRNA and protein, and performed immunohistochemistry at three different levels. Expression was highest in the spinning stage, and in the tissues of head, epidermis and genital organs.