基于寒证动物模型的附子毒性的初步观察

Objective: To observe the effect of syndrome on the toxicity of aconite roots, in order to provide experimental basis for treatment based on syndrome differentiation. Methods: To establish animal model of cold syndrome by ice-water swimming. After swimming, water decoction of crude aconite roots was administrated to mice by gavage, and then LD50 was determined by Bliss method. After swimming, aconitine was administrated to rats by vein injection, and then calculated the duration of arrhythmia. Results: After ice-water swimming, mice and rats appeared cold syndrome, such as weak breathing, shivering, intolerance of cold, preference for swarming, quiet, dull tongue, dull ears, and so on. Compared with the non-swimming mice, LD50 and LD95 of the swimming ones increased slightly. Compared with the non-swimming rats, the duration of arrhythmia of the swimming ones was shorter. Conclusion: Ice-water swimming could simulate cold syndrome to some extent. The toxicity of aconite roots and aconitine to the animals of cold syndrome decreased, which suggested that syndrome and toxicity has correlation.