微沟槽钛表面抗菌肽生物涂层对牙龈成纤维细胞生物学行为的影响

Objective:In this study, GL13k, a cationic antimicrobial peptides, was immobilized onto microgroove surfaces measuring 60 um in width by 10 um in depth via the method of silanization. In vitro cytocompatibility tests, the adhesion and proliferation of HGFs were tested as well as the phenomenon of the contact guidance. Methods:The method of silanization was used to immobilize the antimicrobial peptide GL13K. The cell adhesion, the cell viability, the cell morphology and the phenomenon of the contact guidance were tested by DAPI fluorescence staining, the cell counting kit-8, scanning electron microscopy, and the immuno-fluorescence staining in vitro cytocompatibility tests, respectively. Results: Cell adhesion: the quantitative results showed that the numbers of cells attached to the three different surfaces were not significantly different at the 2 h time point. However, at the 4 h and 6 h time point, the cells attached to the treated and non-treated microgroove surfaces were significantly increased ( P ＜ 0.001) compared with the smooth surfaces. Moreover, significantly higher (P ＜ 0.05) numbers of cells attached to the treated microgroove surfaces than to the non-treated microgroove surfaces at the 6 h time point. Cell viability: At all time points, the cells cultured on the treated microgroove surfaces displayed significantly higher (P ＜ 0.001) viability than those grown on the microgroove surfaces, and even greater than on the smooth surfaces (P ＜ 0.001). The cell morphology and the orientation of the cell growth: HGFs cultured on the treated and non-treated microgroove surfaces were mainly elongated and spindle-shaped and were aligned along the grooves. Meanwhile, the cells cultured on smooth surfaces had a less-defined morphology, some spindle-shaped and some polygon-shaped, and no consistent orientation. Conclusion:In vitro cytocompatibility tests demonstrated that the GL13K-coated microgroove surfaces could promote the adhesion and proliferation of HGFs and guide the growth of the cells along the microgroove.