Cross-reactivity of antibodies against T cell markers in the Bank vole ( Myodes glareolus )
Cross-reactivity of antibodies against T cell markers in the Bank vole ( Myodes glareolus )
Abstract The bank vole is a common Cricetidae rodent that is a reservoir of several zoonotic pathogens and an emerging model in eco-immunology. Here, we add to a developing immunological toolkit for this species by testing the cross-reactivity of commercially available monoclonal antibodies (mAbs) to the bank vole lymphocyte differentiation molecules and a transcription factor. We show that a combination of mAbs against CD4, CD3, and Foxp3 allows flow cytometric distinction of the main subsets of T cells: putative helper CD4+, cytotoxic CD8+ (as CD3+CD4-) and regulatory CD4+Foxp3+. We also provide a comparative analysis of amino acid sequences of CD4, CD8αβ, CD3εγδ and Fopx3 molecules for a number of commonly studied Cricetidae rodents and discuss mAb cross-reactivity patterns reported so far in this rodent family.
Joanna Homa、Magdalena Migalska、Kazimierz Weglarczyk、Katarzyna Baliga-Klimczyk、Renata Mezyk-Kopec
Department of Evolutionary Immunology, Institute of Zoology and Biomedical Research, Faculty of Biology, Jagiellonian UniversityInstitute of Environmental Sciences, Faculty of Biology, Jagiellonian UniversityDepartment of Clinical Immunology, Medical College, Jagiellonian University Department of Clinical Immunology, Institute of Paediatrics, Jagiellonian University Medical CollegeInstitute of Environmental Sciences, Faculty of Biology, Jagiellonian UniversityDepartment of Cell Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University
细胞生物学分子生物学动物学
Myodes glareolusCricetidaeT lymphocytescross-reactivityflow cytometry
Joanna Homa,Magdalena Migalska,Kazimierz Weglarczyk,Katarzyna Baliga-Klimczyk,Renata Mezyk-Kopec.Cross-reactivity of antibodies against T cell markers in the Bank vole ( Myodes glareolus )[EB/OL].(2025-03-28)[2025-08-03].https://www.biorxiv.org/content/10.1101/2023.06.07.544031.点此复制
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