采用FTIR光谱研究小麦胚芽8S球蛋白的二级结构

Wheat germ has a relatively high content of protein, which is composed of albumin and globulin, making it an attractive and promising source of vegetable proteins. Wheat germ 8S globulin was reported the major fraction of wheat germ globulin, hence it is necessary to deeply understand some information about wheat germ 8S globulin. In this work, FTIR spectra were adopted to study the secondary structure of wheat germ 8S globulin. The results of experiments showed that 8S globulin was a protein rich in β-sheet structure (53.8％), followed by α-helical (23.5％) and random coil structure (22.7％).