体内外评价家蚕蛹中30 kDa蛋白群与壳聚糖轭合物对提高蛋白质口服吸收利用度的作用

he oral absorption of peptide drugs is a worldwide problem, in order to increase the degree of oral absorption, we studied in and separated the silkworm pupa powder to find out some special components, our research established the preparation standard of the 30 kDa proteins and chitosan which were made from the silkworm pupa powder, using the conjugates jointed by the 30 kDa proteins and chitosan to wrap the recombiant rhGM-CSF, and fed the mice with the mixture in order to increase the degree of oral absorption, we also let the conjugates wrap with EGFP been digested in the artificial digestion, detected the stability. The result shows by the ELISA detection we found that 15 times higher the amount of rhGM-CSF in the experiment group than in the negative control group; With the fluorescence microscope we found that the EGFP mixed with the conjugates and digested in the artificial digestion(at 37℃) had more stabilize than the EGFP without the conjugates. The summary: our research proved the conjugates(made up with the 30 kDa proteins and chitosan) can increase the degree of oral absorption, and also the proteins which been mixed with the conjugates would avoid from the digestion juice.