生防细菌类芽孢杆菌生物膜形成的影响因子研究

Objective: To make clear whether the initial amount of bacteria, medium composition and co-culture with other strains impact biofilm formation of Paenibacillus or not; Methods: effect of different initial amount of bacteria, different medium, and co-culture on biofilm forming ability of Paenibacillus were measured by the method of crystal violet staining; Result: bacterial growth and biofilm formation increased with the increase of the concentration of starting culture withtin a certain concentration. Furthermore, in general, rice seeds leaching solution is in favor of biofilm formation, however, biofilm formation was significantly inhibited when Paenibacillus were cocultured with other bacteria regardless of biocontrol or pathogenic species; Conclusion: the initial amount of bacteria, the rice seed leaching solution and bacterial co-culture may play an important role in the bacterial growth and biofilm formation of Paenibacillus.