大豆GmTFIIB基因的克隆及其功能预测分析

Soybean mosaic virus (SMV) is a prevalent virus disease in Chinese main soybean producting region(Heilongjiang Province)，which seriously impacts on yield and quality of soybean. Resistance disease gene engineering breeding is one of the effective ways to control the threat to soybean production in Heilongjiang province. In the present study, the GmTFIIB gene was selected as the candidate gene with resistance to soybean mosaic virus, which was based on the fine mapping result of the previous studies. Based on the known CDS sequences of this gene, the complete CDS was clonin, which was 573bp. The homologous sequences of this gene were analyzed, the result showed that Glyma09g11870, Glyma13g25410, Glyma13g25765 and Glyma13g25430 have higher homology with this gene. It suggested that this gene in soybean evolution didn't occur in obvious functional differentiation. The result of tissue specific expression analysis showed that this gene could express in different tissues including roots, stems, leaves, pods, immature fruit, and mature fruit. And this gene could express higher in stems, leaves and immature fruits than that in other tissues. Expression of this gene could be induced by soybean mosaic virus strain N1, which suggested that this gene may be involved in resistance to soybean mosaic virus.