龚弘娟 刘平平 莫权辉 王发明 蒋桥生 叶开玉 齐贝贝 李洁维
作者信息
摘要
现有主流猕猴桃品种的遗传背景相对单一，亲本来源地理分布狭窄，亲缘关系不清晰。为充分利用杂种优势，该研究以广西植物研究所猕猴桃种质资源圃收集的53个猕猴桃品种（品系）叶片为材料，使用SCoT分子标记，进行遗传多样性分析。结果表明：10 条引物在53份猕猴桃供试材料中共扩增出110 条带，各引物扩增的条带在 8~15 条之间，引物平均扩增条带数为 11 条；其中多态性条带 101 条，引物平均扩增多态性条带数为10.1 条，多态性比例为 91.81%。聚类分析显示猕猴桃品种（品系）没有按类型、倍性或选育地等形成明显有规律的聚类关系。但相对来说，同一杂交后代个体之间的亲缘关系比亲本与后代个体之间的亲缘关系更近；芽变品种与原品种并没有表现出特别近的遗传距离，说明芽变材料的突变可能在基因组或染色体层面发生了较大范围的重组、复制或丢失；‘楚红’‘桂红’‘湘吉红’和‘龙藏红’4个红肉品种与‘红阳’亲缘关系明显较远，说明其可能由不同亲本衍生而来；初步验证了 ‘桂海四号’可能为 ‘Hort16A’亲本之一的推测。
Abstract
The genetic background of the existing mainstream kiwifruit varieties is relatively single, the geographical distribution of parental origin is narrow, the relationship is not clear. In order to make full use of heterosis, the leaves of 53 kiwi varieties (strains) collected from the germplasm resource garden of Guangxi Institute of Botanical were used as materials in this study. SCoT molecular marker was used for genetic diversity analysis. The results showed that : article 10 primers kiwi in 53 of the communist party of China, the amplification out 110 bands, each primer amplification bands between 8-15, amplification bands per primer for 11; Article 101 polymorphism bands, article amplification polymorphism bands per primer was 10.1, the polymorphism proportion is 91.81%, the results show that kiwi fruit varieties (strains) not by type, times or breeding ground form such as obvious regular clustering relations. But relatively speaking, the same hybrids individual than the genetic relationship between parent and offspring closer relationships between individuals; Bud mutation varieties as the original does not appear very close genetic distance, that bud mutation material mutations could occur in the genome or chromosome level for major restructuring, duplicate or missing; Chuhong GuihongXiangjihong and Longzanghong four varieties of red meat and theHongyang significantly more distant relatives, it may be derived from different parent; Preliminary validation Guihai No.4 may be Hort16A is one of the parents.关键词
猕猴桃品种/中华猕猴桃/亲缘关系/SCoT分子标记引用本文复制引用
龚弘娟,刘平平,莫权辉,王发明,蒋桥生,叶开玉,齐贝贝,李洁维.53个猕猴桃品种（品系）亲缘关系的SCoT分析[EB/OL].(2021-07-20)[2026-04-05].https://chinaxiv.org/abs/202107.00058.学科分类
农业科学研究/植物学/遗传学
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