斜纹夜蛾SlGNBPⅡ基因的克隆及其功能的RNAi研究

Gram-negative bacteria binding proteins (GNBPs) are a class of PRRs that can recognize LPS and β-1,3-glucans, and have been shown to be involved in activating the Toll and Imd pathways .In this paper, The cDNA encoding Gram-negative bacterial binding protein Ⅱ (GNBPⅡ) was isolated from Spodoptera litura (SlGNBPⅡ,GenBank Accession No.AEQ33590) by homology cloning and rapid amplification of cDNA ends (RACE). The open reading frame (ORF) of SlGNBPⅡwas 1431bp encoding 476 amino acid residues with the predicted molecular weight of 52.36 kDa and pI of 5.59 respectivey. In order to elucify the the fucntion of SlGNBPⅡin vivo of the S. litura.In the paper ,3 μg of dsRNA was injection into the 3rd day of the 4th instar of S. litura. The result of Real-time PCR showed that the mRNA expression level of SlGNBPⅡ decreased after dsRNA microinjection in 4hr,6hr,12hr,24hr.In order to further prove whether SlGNBPⅡwas knowdown by RNAi , the downstream antimicrobial peptides were used to check ,such as Cecropin A,Cecropin B and moricin, the semi--quantitative PCR results show that the mRNA expression level of Cecropin A,Cecropin A and moricin was desrease after the 24 hr. All the above results lay a basis on the molecular mechanism between SlGNBPⅡ and the innate immunity of S. litura.