胰岛素、催乳素对奶山羊乳腺上皮细胞泌乳功能的影响

Mammary gland epithelial cells which have lactation function can be used in assessment of mammary gland expression vector; it can also be a cell mode of mammary gland development and lactation regulation mechanism. We assess the lactation functional of dairy goat mammary epithelial cell line. Results showed that mammary gland epithelial cells could excrete milk fat, milk protein and lactose in fluid DF12 with addition of EGF, IGF-1, ITS and FBS. Lactation function mammary gland cells have no obvious changes after insulin treatment 48h; 48h of prolactin treatment can obviously improves the secretion of milk proteins and lactose.