Jagged-1抑制Th17细胞分化的机制初探

A soluble Jagged-1/Fc chimera protein (Jagged-1) was directly used to activate Jagged-1-Notch signaling to investigate effect of Jagged-1 on the differentiation CD4+ T cells into Th17 cells. CD4+ T cells were isolated from mouse lymphocytes using magnetic-activated cell sorting (MACS) and treated with Jagged-1. Flow cytometry was performed to analyse the expression of IL-17A on CD4+ T cells. RT-PCR were employed to calculate the mRNA of Il17a and Il17f. Western blot was used to detect RORγt protein level in CD4+ T cells. ELISA was used to determine the level of IL-17A, IL-17F from the culture supernatant of the cultured CD4+ T cells. The results indicated that the soluble Jagged-1/Fc suppressed CD4+ T cells to differentiate into CD4+IL-17+ cells. The mRNA of Il17a and Il17f were significantly inhibited by Jagged-1. Jagged-1 down-regulated the expression of RORγt protein in CD4+ T cells. Jagged-1 suppressed the secretion of IL-17A and IL-17F in the treated cells. The results suggest Jagged-1 inhibits the skewing of CD4+ T cells toward Th17 cells, associated with suppression the expression of IL-17A, IL-17F and RORγt.