烟草中CycB基因的表达分析
nalysis of the expression of a CycB gene isolated from Nicotiana tabacum
周期蛋白是一类重要的细胞周期调节因子，其过量表达可能会改变植物的生长与发育。在本研究中我们从烟草（Nicotiana tabacum）中克隆了CycB基因。Northern 杂交表明该基因在茎顶端分生组织和花芽中过量表达。为了进一步检测其在植物生长发育中的功能，利用农杆菌介导的方法将该基因转入烟草基因组中，由CaMV 35S强启动子驱动表达。结果表明，过量表达CycB基因的转基因植物表现出与野生型相似的生长速率，同时，流式细胞仪检测的结果也表明在转基因植株和野生型之间并没有明显的区别。相比之下，转基因烟草中CycB的过量表达能促进叶片外植体在无外加激素的MS培养基上产生不定芽及不定根，说明CycB的表达可能与植物细胞的分化有关。
he expression profile of a CycB gene from Nicotiana tabacum was characterized in present study. The results showed this gene exhibited high expression in shoot apex and flower. This CycB gene, driven by CaMV 35S promoter, was introduced into N. tabacum using Agrobacterium-mediated method. However, the transformants overexpressing CycB showed similar phenotype to wild-type plants. By contrast, overexpression of CycB enhanced the adventitious root and shoot formation on leaf explant in transgenic plant on MS medium in the absence of hormone.
娄玲玲、邢晓宇、曲冠证
细胞生物学遗传学植物学
B类周期蛋白Nicotiana tabacum转基因烟草
ycBtransgenic tobaccoNicotiana tabacum
娄玲玲,邢晓宇,曲冠证.烟草中CycB基因的表达分析[EB/OL].(2010-09-28)[2025-05-24].http://www.paper.edu.cn/releasepaper/content/201009-608.点此复制
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