一个与水稻条纹病毒侵染相关的水稻miRNA的功能研究

ImiRNA plays key regulation roles in the plant development. many reports have been reported that viral infection could lead to the change of plant miRNA expression pattern, which was supposed to be a reason of viral symptom development. Meanwhile, there were few reports on what the roles of miRNA expression pattern change are to viral infection. Here, a novel miRNA, nmiR119, identified previous from small RNA libraries of rice stripe virus (RSV)-infected rice by us was focused to understand its function. Results showed that nmiR119 was upregulated in the RSV-infected rice plant, and their two target genes were downregulated correspondingly. Transgenic rice plant overexpressing nmiR119 had the similar phenotype to wild type plant, while the nmiR119-suppressed rice plant showed the extreme low seed rate. In RSV challenge analysis, both nmiR119-overexpressed plant and nmiR119-suppressed plant had the slight higher incidence of disease comparing to the wild type plant. But, the accumulation of RSV RNAs were at a extreme low level, indicating the potential roles of nmiRNA played for viral RNA replication.