刚毛柽柳ThGAI 基因克隆及表达分析

he ThGAI gene was successfully cloned from the transcrip cDNA library of Tamarix hispida . Sequence analysis indicated that is 2901bp in length, containing an open reading frame of 1980bp, encoding 659 amino acids. The estimated molecular weight and isoelectric point of the putative protein were 71.99 kDa and 5.58, respectively. Bioinformatics analysis indicated that the typical domains of DELLA protein were found in the protein encoded by ThGAI gene. Amino acid sequence alignment with other species and phylogenetic analysis indicates that the deduced amino acid sequence of ThGAI gene was highly homologous with those of DELLA protein from other plants，was the number of DELLA .They had the DELLA protein typical domains such as TVHYNP, POLY(S/T),NLS,VHIID and the conserved GRAS domain. They had the highest homology of with ZmGAI from Zea mays Linn. Quantitative real-time PCR assay revealed that the mRNA levels of ThGAI was significantly up-regulated under NaCl and PEG treatments, suggesting that GA may be involved in abiotic stress response of Tamarix hispida.