苦瓜提取物抑制3T3-L1细胞脂肪沉积的初步研究

Obesity is recognized as a low-grade chronic inflammatory disease characterized by lipid accumulation（adipogenesis), causing serious public health problems. Bitter melon (Momordica charantia) has been shown to ameliorate diet-induced obesity, but the molecular mechanisms are still unknown.The objective of this study was to elucidate the effect of bitter melon extracts on adipogenesis in 3T3-L1 adipocytes.The results revealed that bitter melon markedly inhibited lipid accumulation. The n-butanol fraction blocked the expression of adipogenic transcription factors, including peroxisome proliferator-activated receptors (PPARγ), CCAAT/enhancer binding proteins (C/EBPα) and Sterol regulatory element binding protein-1c (SREBP-1c). Morover, down-regulated the mRNA levels of 5 key lipogenic enzyme gene expression, including FAS、GLUT4、ACC1、Fasn、aP2 and LPL. These results indicate that n-butanol fraction of bitter melon suppresses adipocyte differentiation and lipid accumulation through multiple mechanisms and may have applications for the treatment of obesity.