体外环境下纳米细菌与大肠杆菌促结石的协同作用

Objectives: The purpose of this study is to discover the evidence of nanobacteria and Escherichia coli interaction and coordination of stone formation in vitro and to offer a new research field about the formation of the gallbladder stone. Methods: Nanobacteria and Escherichia coli were mixed cultured and then identified by Dahl McGec-Russel calcium-alizarin red staining, observed by electronic microscope and determined by β-glucuronidase, to find out the path-physiologic mechanism of their interaction. Results: The mixed culture of the nanobacteria and the Escherichia coli led to the mineralization of the Escherichia coli which were positive with calcific staining. The mixed culture could not lead to an extra excretion of β-glucuronidase in Escherichia coli. Conclusions: Nanobacteria is a novel apatite mineral-forming bacteria which can lead to the mineralization of Escherichia coli in mixed culture. There is no sign that nanobacteria can promote the secretory of enzyme β-glucuronidase in Escherichia coli, which is helpful to the formation of gallbladder stone.