重复经颅磁刺激对阿尔茨海默病学习记忆功能的预保护研究

Objective: By the methods of ethology, electrophysiology and ultramicropathology， from the view of both Neurology and Neuroinformatics, on micro- and macro-levels of the neural system, the effects of preventing rats from AD were studied via repetitive transcranial magnetic stimulation. Methods: Altogether 4 groups ( 12 rats for each group)involved. 2 of 4 were given low-frequency rTMS, high-frequency rTMS respectively as non-medicine pre-intervention, 1 of 4 as control, 1 of 4 for AD models directly. In this study, rat models were established by co-injecting Aβand Ibo into bilateral nucleus basalis of Meynert. Morris water maze test, ultramicropathology in hippocampus CA3 area were measured before and after co-injection for AD model. Results:1.AD rat models were successfully estebilshed with severe learning and memory defects by co-injecting Aβand Ibo into bilateral nucleus basalis of Meynert. 2. The direct AD model group got worse results in Morris and ultramicropathology tests in CA3 area of Hippocampus. 3. rTMS was effective to help rats keep their learning and memory abilities. Low frequency rTMS was more effective than high frequency one to prevent rats from AD. Conclusion: The AD rat models established by co-injecting Aβand Ibo into bilateral nucleus basalis of Meynet can simulate AD’s severe learning and memory impairments. This kind of AD model is more suitable for the study of AD prevention. rTMS including both in low and high frequency is all effective to prevent rats from AD. Furthermore, low frequency rTMS has more effects than high frequency rTMS does on the neurons, synapses as well as learning and memory abilities caused by co-injecting Aβand Ibo for AD model’s being established.