复合酶催化大豆油水解的研究

Reaction conditions for compound lipase catalyzed hydrolysis of soybean oil were investigated. Five different source lipases of were selected to investigate the complex effect. Combining C. lipolytica and Amano-G could give a better hydrolysis rate and yield. The hydrolysis reaction could be accelerated by ultrasound, especially in the initial reaction stage. The optimum reaction condition was obtained: the concentration of C. lipolytica and Amano-G were 1%(w/w) and 0.1%(w/w), respectively; adding Amano-G after C. lipolytica acted 1h; temperature was 45℃; water-phase was de-ionized water, and water/oil ratio was 1:1(w/w). Under above condition, reaction yield for 24h could be enhanced from 45.7% in conventional shaking bath to 94.2% catalyzed by ultrasonic-bi-enzyme.