利用基因芯片分析TuMV侵染对白菜分泌途径基因的影响
Microarray Analysis of the Secretory Pathway Genes Response to TuMV Infection in Brassica rapa
前人研究发现病毒的复制复合体（viral RNP complex）和病毒RNA通过分泌途径移动到胞间连丝。本研究通过拟南芥基因芯片鉴定病原物侵染引起表达变化的基因，结果表明，38个分泌途径基因包括AP1, AP2, B-COP, F-COP, Sec23/24, ER-Golgi, PM2, PM and TGN/PM。qRT-PCR验证表明基因表达情况与基因芯片结果一致。这些结果表明病毒利用分泌途径的基因完成病毒的复制和运输。对于这些基因的进一步研究和分析有助于发现新的抗病基因。
Previous evidence suggested the viral RNP complex consisting of the viral RNA moved to plasmodesmata via the secretory pathway. This global analysis of Arabidopsis gene expression identifys a complex array of changes impacting pathogen response. 38 secretory pathway genes are identified, including AP1, AP2, B-COP, F-COP, Sec23/24, ER-Golgi, PM2, PM and TGN/PM. The qRT-PCR is basically consistent with microarray analysis. All these results suggest viruses use the secretory pathway to accomplish the viral replication and movement. Exploring the precise function of the new genes in this analysis will offer new insights into the defense in Brassica rapa.
张昌伟、李彦肖
分子生物学植物学遗传学
基因芯片分泌途径芜菁花叶病毒
microarray analysissecretory pathwayTuMV
张昌伟,李彦肖.利用基因芯片分析TuMV侵染对白菜分泌途径基因的影响[EB/OL].(2014-06-25)[2025-08-02].http://www.paper.edu.cn/releasepaper/content/201406-402.点此复制
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