大豆F3H基因InDel标记的开发及定位

In order to develop and map F3H gene, a key enzymes in isoflavone synthesis pathway of soybean, a recombinant inbred line (RIL) population, NJRIKY, which was derived from the cross Kefeng 1 ×Nannong 1138-2, was used to constructed the genetic linkage map. Using gene specific primer pair designed according to the sequence of F3H gene from soybean, a fragment about of 2600 bp was generated. Sequencing of fragments revealed there were 3bp insertion/deletion（InDel）differences among the parents of NJRIKY, and an InDel marker was developed for F3H gene based on the sequence variation. The InDel marker, namely F3H gene was mapped on linkage group G8-D1b+W by PCR amplification detection in the RIL population NJRIKY. The F3H gene and Rsc-7 locus, which is resistant to SMV, were mapped on the same linkage group and located to between Satt698 and Sat_351 of SSR makers with distances of 38.5 and 9.4 cM, respectively.