不同氧化修饰的酪蛋白对小鼠消化器官的氧化损伤
Oxidative damage of oxidized casein to mice digestive organs
目的：本文主要评估H2O2-Cu及脂质氧化产物（MDA）氧化修饰的酪蛋白对小鼠消化器官氧化还原状态的影响。方法：用H2O2-Cu、 MDA处理酪蛋白，测定蛋白体外蛋白消化率及消化产物清除自由基能力。饲喂小鼠10周，测定小鼠消化器官的抗氧化及氧化损伤指标。结果：酪蛋白经过两种氧化处理后，羰基、双酪氨酸（Dtyr）、晚期氧化蛋白产物（AOPP）含量均呈上升趋势，巯基含量下降。氧化蛋白体外消化率下降，消化液清除自由基能力下降。饲喂氧化酪蛋白日粮组小鼠的消化器官氧化还原状态失衡，谷胱甘肽过氧化物酶（GSH-Px），过氧化氢还原酶（CAT）活性及总体抗氧化 (T-AOC)能力下降，活性氧（ROS）水平上升，并积累大量氧化蛋白产物，包括羰基、MDA、Dtyr、AOPP。结论：氧化导致酪蛋白消化率下降，蛋白消化液清除自由基能力下降，摄入氧化酪蛋白会引起机体的氧化应激，降低机体抗氧化能力，并导致消化器官的氧化损伤。
Objective: The objective of this work was to evaluate the impacts of oxidized casein oxidized by H2O2-Cu and MDA on redox state of mice digestive organs. Methods: Casein was oxidized by H2O2 combined with Cu2+(H2O2-Cu) and MDA, then casein digestibility in vitro and free radical scavenging ability of casein digestive juice were measured. After long-term feeding, oxidative damage indicators of mice digestive organs, including liver, pancreas and jejunum were detected. Results: After oxidation, contents of advanced oxidation protein products (AOPPs), dityrosine (Dtyr) and carbonyl in oxidized casein increased, while thiol content decreased. Protein digestibility and free radical scavenging capacity of protein digestive juice all decreased. After long-term feeding, redox state imbalance of mice digestive organs was identified by the increased contents of ROS, carbonyl, MDA, Dtyr and AOPP (P<0.05), and the significant decreased levels of GSH-Px, CAT and total antioxidant capacity (T-AOC) (P<0.05) in oxidized casein group. Conclusion: Oxidized casein could cause oxidative stress and accumulation of protein oxidation products in mice digestive organs.
乐国伟、施用晖、吴伶燕、李竹青
生物化学生理学细胞生物学
氧化酪蛋白消化器官氧化损伤羰基tyrOPP
oxidized-caseindigestive organsoxidative damagecarbonylDtyrAOPP
乐国伟,施用晖,吴伶燕,李竹青.不同氧化修饰的酪蛋白对小鼠消化器官的氧化损伤[EB/OL].(2012-03-23)[2025-08-11].http://www.paper.edu.cn/releasepaper/content/201203-699.点此复制
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