推拿对坐骨神经损伤大鼠胶质细胞源性神经营养因子的影响

【Objective】This study is based on the good clinical effect of Tuina treatment in peripheral nerve injuries, in order to confirm the ameliorative effects of Tuina treament on the sensory disturbance and motor dysfunction caused by peripheral nerve injuries, meanwhile interpret the central mechanism of Tuina on the restoration of peripheral nerve injury. 【Methods】Selecting the sciatic nerve injured rats treated by Tuina treatment, observing the spinal cord which are stained with HE staining by the optical microscope; analyzing the spinal cord which are stained with immunohistochemical staining by the the optical microscope, the glial-cell-line derived nerotrophic factor(GDNF) is chosed as the basic point from neuro trophic factors which can promote the nerve regeneration, to provide theoretical support for revealing the the central mechanism of Tuina treament on the restoration of peripheral nerve injury. 【Results】In view of HE staining of spinal cord, the integrity and the directivity of the spinal cord cell arrangement in Tuina treatment group is better than model group and control group, cell nuclear is locating in the center, cell regeneration can be seen, axons are extending to the distal part, that is indicating that Tuina treatment can promote the regeneration of spinal cord anterior horn motor neurons after peripheral nerve injuries. The GDNF in cytoplasm of spinal cord neurons has positive expression after stained,it is the more uniform brown granules, the average optical density of GDNF in Tuina treatment group is higher than model group and control group, thus improving that Tuinatreament can promote the nerve regeneration by increased the content of GDNF in spinal cord.【Conclusion】Comprehensive analyze the result of histomor phology and immunohistochemical staining, according to preliminary inference, the mechanism ameliorative effects of Tuina treament on the sensory disturbance and motor dysfunction caused by peripheral nerve injuries is promoting the expression of GDNF in spinal cord.