水稻转录因子WRKY-P1对磷素积累的影响

n increasing number of genes have been reported to play pivotal roles in the phosphate starvation signaling network in rice. However, few attentions have been paid to rice WRKY transcription factors involved in this network. In order to elucidate the functions and regulatory mechanisms of rice WRKY transription factors in phosphate starvation responses, a phosphate starvation-responsive WRKY transcription factor, OsWRKY-P1, was identified in this work. By transgenic approaches and RT-qPCR analysis, the subcellular localization, physiological parameters and the regulation of the downstream phosphate starvation-induced genes were investigated in the overexpression lines. The results showed that both the soluble phosphate concentration was increased in the Pi-replete OsWRKY-P1 overexpression lines as compared with the wild-type plants. Unexpectedly, only one phosphate transporter gene of the PHT1 family, OsPHT1;12, which has a extremely low expression level, was upregulated upon OsWRKY-P1 overexpression. Taken together, OsWRKY-P1 is involved in phosphate starvation responses, and the underlying molecular mechanisms need to be further explored.