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首页|日粮乳果糖通过提高雌马酚水平改善LPS诱导状态下仔猪体液免疫、肝脏氧化性能并改变结肠微生物区系

日粮乳果糖通过提高雌马酚水平改善LPS诱导状态下仔猪体液免疫、肝脏氧化性能并改变结肠微生物区系

ietary lactulose enhances humoral immunity, alleviates liver oxidant and shifts colon microbiota by improving equol transformation in barrows challenged with Escherichia coli lipopolysaccharide

中文摘要英文摘要

试验选用36头40 d去势公仔猪,随机分为三组;分别饲喂无豆对照料,添加50 mg/kg大豆苷原料,添加50 mg/kg大豆苷原+1%乳果糖料。饲养23天后,每组随机选取6头进行LPS攻毒(25 μg/kg BW)。结果表明,在免疫应激条件下,饲喂大豆苷原能显著增加外周血T和B淋巴细胞转化率,而乳果糖+大豆苷原组的T和B淋巴细胞转化率又显著高于大豆苷原组;血液中白细胞介素和激素水平结果显示,对照组的白细胞介素6(IL-6)水平显著高于大豆苷原组和乳果糖+大豆苷原组(P<0.05);大豆苷原组和乳果糖+大豆苷原组血液中皮质醇水平显著高于对照组(P<0.05)。大豆苷原能显著降低肝脏中总羟基、NO、8-羟基脱氧鸟苷和丙二醛水平,但乳果糖+大豆苷原没能促进对以上氧化标记物的抑制作用;此外大豆苷原显著降低了肝脏中T-SOD、CuZn-SOD、Mn-SOD及谷胱甘肽还原酶(GR)的活性,而乳果糖+大豆苷原T-SOD、Mn-SOD和谷胱甘肽还原酶(GR)活性显著高于大豆苷原组。此外,乳果糖能显著改善结肠微生物的群落和数量。

In this paper the effects of dietary daidzein alone or in combination with lactulose on humoral immunity, liver oxidant status and colon microbiota of barrows (Landrace×Duroc) challenged with Escherichia coli lipopolysaccharide (LPS). A total of 36 barrows (age, 40 d; initial BW, 9.35±0.17 kg) were randomly allotted into 6 pens (6 pigs/pen; 2 pens/treatment). Dietary treatments were: 1) control (C, n=12, fed an isoflavones-free basic diet), 2) control+daidzein (D, n=12, fed an isoflavones-free basic diet with 50mg/kg of daidzein supplementation), and 3) control+daidzein+lactulose group (D+L, n=12, fed an isoflavones-free basic diet with 1% of lactulose and 50mg/kg of daidzein supplementation). After 23 days, 6 of pigs from each treatment were randomly selected and injected with LPS. After 24 h post injection, dietary daidzein was found significantly increased the peripheral blood lymphocyte proliferation response to both and PHA and LPS stimulation, and daidzein combination with lactulose supplementation had higher response. After 48 h post injection, pigs were killed. In the D and D+L groups, plasma IL-6 level was lower than C group, while cortisol concentration was higher. Furthermore, dietary daidzein or combination with lactulose may improve the profiles of colonic microflora, and maintains the bacteria populations, including hydrogen-utilizing bacteria after LPS challenge. Moreover, by adding lactulose can alleviates the liver oxidative stress caused by LPS challenge in barrows treated with daidzein.

姚文、郑卫江

农业科学研究畜牧、动物医学生物科学现状、生物科学发展

动物营养乳果糖雌马酚体液免疫抗氧化结肠微生物

Animal nutritionLactuloseEquolHumoral ImmunityAnti-oxidantColon Microbiota

姚文,郑卫江.日粮乳果糖通过提高雌马酚水平改善LPS诱导状态下仔猪体液免疫、肝脏氧化性能并改变结肠微生物区系[EB/OL].(2015-11-27)[2025-08-18].http://www.paper.edu.cn/releasepaper/content/201511-707.点此复制

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