黄麻SSR标记开发、遗传图谱构建与株高QTL定位

Jute, comprising white and dark jute, is one of the most important crops for natural ?ber production. In this study, a set of 197 novel SSRs from 529 publicly available dark jute genomic sequences were developed and used to assess polymorphism among six diverse accessions of dark and white jute. Of these SSRs, 142 (72.1%) successfully amplified at least one fragment and 122 (61.9%) detected polymorphism, showing the high transferability of these dark jute SSRs to white jute.An SSR genetic map of white jute using an F8 recombinant inbred lines population consisting of 94 lines derived from a cross between Huangma 179 and Aidianyehuangma was constructed. The map, comprising 39 newly developed SSR markers and spanning 389.9 cM with an average distance of 12.1cM between adjacent markers, was used for identifying quantitative trait loci (QTL) for plant height. Six QTL were detected, of which qPH.N1 was stable in different environments and regarded as a major QTL. This study has not only enriched the stock of jute SSR markers but also shed light on the genetic basisof plant height at the QTL level in jute.