结肠癌转移前后基因表达数据分析

Gene expression in the SW480 primary tumor colon cancer cell line was compared to that in the SW620 metastatic colon cancer cell line. Using filtering criteria of a 1.5 or greater fold-change in expression, 1554 out of over 18,000 genes were differentially expressed. The expression of 1034 genes was increased in the metastatic cell line, while 520 showed decreased expression in the metastatic cells. The Gene Ontology term analysis identified an over-representation of genes involved in telomerase dependent telomere maintenance among the up-regulated genes and an over-representation of genes involved in cell matrix adhesion among the down regulated genes. The analysis of KEGG pathway terms identified a significant over representation of cell cycle genes among the up-regulated genes. The increased expression of genes involved in cell cycle and telomere maintenance, as well as the decreased expression of genes involved in cell matrix adhesion, may contribute to the progression to metastasis in colon cancer cells.