亚洲栽培稻与非洲栽培稻杂交种间杂种花粉不育基因的精细定位
Fine mapping of hybrid pollen sterility gene between Oryza saliva L. and Oryza glaberrima S.
栽培稻分为亚洲栽培稻（Oryza sativa L., 2n =2x= 24）和非洲栽培稻（O. glaberrima Steud, 2n = 2x= 24）两个栽培种，非洲栽培稻种资源中广泛存在着各种抗病虫、耐盐碱、抗旱、耐淹、耐高温等相关性状的优良基因，但由于栽培稻种间杂种的高度不育，严重限制了非洲栽培稻有利基因向亚洲栽培稻转移及栽培稻种间杂种优势的利用。为阐明亚洲栽培稻与非洲栽培稻种间杂种不育的机理和进一步利用种间杂种优势，本研究以滇粳优1号（DJY1）为受体亲本，非洲栽培稻IRGC102295为供体亲本，构建了一个近等基因系NIL，滇粳优1号与NIL杂种F1花粉表现典型的半不育，败育类型为染败，受一对杂合基因座位S37控制。利用743株滇粳优1号与NIL杂交F2群体进行连锁分析，初步将S37限定在第12染色体短臂末端的两标记NJ5和G4之间，随后利用扩大的18014株F2群体，最终将S37精细定位到标记HP14和G21之间，物理距离为73 kb。为最终打破非洲栽培稻和亚洲栽培稻之间的生殖隔离，筛选出具有优良性状的水稻新种质创造了条件。
IWe developed an NIL via repeated backcrossing and molecular marker-assisted selection (MAS), where the japonica variety Dianjingyou 1 was used as the receptor parent and O. glaberrima Steud variety IRGC102203 as the donor parent. F1 pollen fertility of NIL/DJY1 exhibited typical semi-sterility, and the type of pollen abortion exhibited stained abortive. At the same time, we examined the DJY1 and F1 pollens using scanning electron microscopy and transmission electron microscopy and found the stained abortive pollens in F1 hybrid have small volume and most of them are shrunken, which have no accumulations of starch granules. According to pollen fertility, we constructed a linkage map covering the S37 region by 743 plants randomly selected from F2 progenies. The results indicated that the S37 locus was located to 2.2 cM in the interval between NJ5 and G4. Finally, S37 was mapped to the region between HP14 and G21 using a large F2 population contained 18014 plants, with physical size of about 73 kb.
赵志刚、王超龙、万建民、汪勇
农业科学研究遗传学植物学
花粉不育分子标记精细定位水稻
Pollen sterilitymolecular markerFine mappingrice
赵志刚,王超龙,万建民,汪勇.亚洲栽培稻与非洲栽培稻杂交种间杂种花粉不育基因的精细定位[EB/OL].(2012-02-16)[2025-08-11].http://www.paper.edu.cn/releasepaper/content/201202-533.点此复制
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