激素配比对白桦悬浮细胞中三萜积累的影响

allus were induced by birch stems on IS medium and suspension cells were used to study the effect of the types and concentrations of plant hormones on the relation between the growth situation and the content of triterpenoids. During one suspension culture cycle of birch, the cell viability and triterpenoid content of suspension cell reached the maximum after 9 days, and the values were 1.42 and 14.31 mg • g-1 (DW), respectively. These results suggest that suspension culture liquid replacement was every 7~10 d. On the hormone combinations between 6-BA + NAA and 6-BA + TDZ, 6-BA与TDZ were beneficial to accumulation of cell dry weight and triterpenoids. Among of them, 0.6mg•L-1 TDZ +0.2 mg•L-1 6-BA was best to increase of dry cell weight and triterpenoid yield, the suspension cell weight and triterpenoid yield cultured after 25d was 0.99 g•bottle-1 and 0.22 g•L-1, respectively; 0.4 mg•L-1 6-BA +0.2 mg•L-1TDZ was best to accumulation of triterpenoids, the content and yiled of triterpenoids cultured after 25d was 14.23mg•g-1and 0.21 g•L-1, respectively. It was concluded from above results that the best culture condintion for triterpenoid accumulation was 0.4 mg•L-1 6-BA +0.2 mg•L-1TDZ.