抑制NGF的促血管生成作用对神经再生的影响

o explore the relation of NGF on the angiogenesis and neurogenesis. The neurogenesis in SVZ and SGZ are investigated with Brdu labelling through suppression of the phosphoralation of FAK. Taken intervention animal model of cerebral ischemia- reperfusion. Male SD rats are randomly divided into 4 groups: sham operation group, cerebral ischemia group, NGF group, NGF+TAE226 group. Immunoflurorescence histochemistry are employed to detect the cell proliferation and differentiation in SVZ and SGZ. The numbers of Brdu positive cells and the expression of DCX in SVZ and SGZ of the NGF group, particularly more than other 3 groups. The double-labeling cell numbers of Brdu and DCX protein are more in the group of NGF than NGF+TAE226. The increased neurogenesis by NGF after cerebral ischemia is maybe correlated with the increased angiogenesis caused by NGF.