单级自养脱氮系统微生物群落结构研究

wo sets of the one-step completely autotrophic nitrogen removal process were set up. To analyze the microbial community structure, active sludge and biofilm samples were taken from these two single sequencing batch biofilm reactors (SBBR).Reactor A which was filled with hollow balls and flexible packing materials runs with a high nitrogen removal efficiency. The similarity of microbial community structure between active sludge and biofilm samples is 58.3%. The characters of DO distributing on active sludge and biofilm make a suitable condition for different bacteria. Their co-metabolisms make the ammonium oxidation and TN removal rate reach to 90% and 80% respectively. Reactor B which was only filled with flexible packing materials works under a low efficiency. The concentration of biofilm is low. There is not much difference between biofilm and active sludge samples on the microbial community structure. The similarity reaches to 100%. Although bacterial richness value of sample B is high, the variety of dominant bacteria is poor. TN removal rate is only about 10% to 20%. Biofilm is one of the key factors of the one-step completely autotrophic nitrogen removal process under high DO.