沪杭地区番茄上黄瓜花叶病毒基因多态性的研究

ucumber mosaic virus (CMV) and Tomato mosaic virus (ToMV) have been considered as the main viruses on tomatoes in China. Single or mixed infections of the two above viruses are often found. And virus species, region and climate are relevant to the occurrence of viral disease. In this study, tomato leaves with symptoms were collected in summer and autumn in Eastern China especially Shanghai and Hangzhou. A series of methods were used to detect and differentiate viruses including host diversity, multiplex RT-PCR, restriction enzyme analysis, SPAT, homology comparison and phylogenetic tree analysis. Results showed that CMV and ToMV were found to be the principal viruses infected tomato crops in Eastern China. Both single and mixed infections were found by multiplex RT-PCR in the study. CMV was found to more commonly distribute than ToMV, CMV Ⅰwas predominant while CMV Ⅱshowed potential rise in Eastern China. CMV ⅠB was more prevail than CMV ⅠA and sat RNA parasitized in CMV subgroupⅠ was also found using multiplex RT-PCR. In additon，no reassortant was detected in our work.