芪参益气滴丸对气虚血瘀证心肌梗死大鼠的心肌保护机制

In this paper,to observe the protection and its mechanism of Qishenyiqi dripping pills on .Methods: 50 male SD rats were randomly divided into normal group, model control group, experimental model group, isosorbide dinitrate(ISD) group, Qishen Yiqi(QSYQ)group, 10 rats in each group. Establishment of myocardial infarction models and the deficiency of Qi and blood stasis myocardial infarction model, respectively, with isosorbide dinitrate and Shenqi Yiqi Drop Pill intervention. Cardiac ejection fraction, myocardial infarction and myocardial tissue GSK-3beta, TRL4,NF-κB,beta-catenin protein and gene expression were observated. Results: After NBT staining, myocardial tissue in the normal group were stained purple; There were large grey infarcted region in myocardial tissue 0f each model group. There were smaller gray infarction area than ISD group and QSYQ group. The model group, ejection fraction (EF) significantly lower than the normal group; Qishen Yiqi group could improve cardiac function(P<0.01). Immunohistochemical results shown, the normal group GSK-3 beta and of TRL4 protein expression was low, the model group were higher than those in normal group(P<0.01); isosorbide dinitrate and Shenqi Yiqi Drop Pill could increase GSK-3 beta and TRL4 protein expression (P<0.01).RT-PCR results show, beta-catenin gene expression level was s the highest and NF-κB gene level was lowest in the normal group, the model group beta-catenin gene level was lower than that in normal group and NF-κB gene level was higher than that in normal group (P<0.01); Qishen Yiqi Drop Pill could increase beta-catenin gene expression and decrease NF-κB gene level (<0.01). Conclusion The effect of Qishen Yiqi Drop Pill on myocardial protection may be relate to regulation of TRL4 / NF-κB, GSK-3β/β-Catenin pathway .