结外鼻型NK/T细胞淋巴瘤与T细胞淋巴瘤/白血病细胞株间差异蛋白表达的分析

o compare the differently-expressed proteins between ENKTCL-N and T cell lymphoma/leukemia, which was helpful in understanding the characteristics of ENKTCL-N protein expression, finding the characteristic markers for ENKTCL-N to make differetiat diagnosis, and making a foundation for further research of the pathogenesis and progression of ENKTCL-N in the protein level. The differently-expressed proteins had been isolated by 2-DE, compared and analyzed by software and by handwork, and identified by mass spectrometry (MS）between ENKTCL-N cell line SNK6 and T cell lymphoma/leukemia cell line Hut78. The 2-DE comparison results of ENKTCL-N cell line SNK6 and T cell lymphoma/leukemia cell line Hut78 showed that the match rate of the two cell lines was 72.15%, which suggested that there were many similarities between ENKTCL-N cell line SNK6 and T cell lymphoma/leukemia Hut78. Fourteen pairs of 5-fold different protein spots had been detected, from which 10 proteins had been identified by MS and protein-database. In Hut78 cell line, four proteins were up-regulated, four proteins were newly-appeared and two disappeared. The differently-expressed proteins between ENKTCL-N cell line SNK6 and T cell lymphoma/leukemia cell lines were mainly involved cell differentiation, and proliferation, anti-apoptosis, angiogenesis, EB virus infection, chemotherapy resistance, and so on, which was helpful in trying to find the special markers between ENKTCL-N and T cell lymphoma/leukemia, and set up an essential foundation for deep research of the pathogenesis and progression of ENKTCL-N in the protein level.