环氧树脂基液相色谱整体柱的制备及性能研究
Synthesis and characteristics of epoxy resin-based liquid chromatographic monolithic column
基于原位逐步聚合反应，将质量比为4: 1: 8的环氧树脂、二乙烯三胺和聚乙二醇（PEG-1540）均匀混合后，灌入不锈钢色谱空管柱(50mm×4.6mm i.d) 内，两端密封于60 C反应24h，经过后处理得到一种新型环氧树脂基聚合物液相色谱整体柱，并利用扫描电镜和和红外光谱进行了初步表征。以烟酸和烟酰胺为模型分析物，考察了流速、流动相对在整体柱上保留和分离的影响；在室温下，100% 纯水流动相，流速为1.0 mLomin-1时，烟酸和烟酰胺可以在色谱柱上2min 内快速分离，分离度为 0.83。
novel epoxy resin-based polymer monolithic column is prepared from PEG-1540, epoxy resin, diethlenetriamine with the mass ratio is 8:4:1 in the blank stainless steel chromatographic columns (50mm×4.6mm i.d) by in-situ step-addition polymerization. The columns have been characterized by scanning electron microscopy and FT-IR. The chromatographic performance of nicotinamide and nicotinic acid on the monolithic column is evaluated. The content of flow rate and organic solvent were investigated in the experiment. Rapid separation of nicotinamide and nicotinic acid is achieved with 100% pure water as mobile phase in 2.0 min at flow rate of 1.0 mLomin-1. The resolution is 0.83.
王邃、李海龙、陶艳玲
高分子化合物工业合成树脂工业、塑料工业有机化学工业
整体柱固定相高效液相色谱环氧树脂
Monolithic columnStationary phaseHigh performance liquid chromatography (HPLC)Epoxy resin
王邃,李海龙,陶艳玲.环氧树脂基液相色谱整体柱的制备及性能研究[EB/OL].(2011-05-26)[2025-08-11].http://www.paper.edu.cn/releasepaper/content/201105-612.点此复制
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