二茂铁基谷胱甘肽修饰电极的制备及其对痕量镉离子的检测
Ferrocenoyl Glutathione Modified Electrodes: Fabrication and Application for Detection of Cadmium Ions
首次以二茂铁甲酸和还原型的谷胱甘肽（GSH）为原料，合成了二茂铁基谷胱甘肽（1’-cysteaminecarbonyl-1-glutathionecarbonyl-ferrocene ，简称Fc-GSH) 。用IR和1H-NMR对中间物和产物进行了表征。通过自组装将Fc-GSH单分子膜（SAM）固定在金电极的表面，制备了Fc-GSH 修饰电极。用循环伏安法(CV) 研究了在含Cd2+的水溶液中修饰电极的电化学特性。研究发现，溶液中Cd2+的存在，使Fc-GSH 的氧化峰电位产生正移以及峰电位差变大；而且当溶液中的Cd2+的浓度小于20 nM时，这种正移的值与溶液中的Cd2+的浓度成正比。因此可以利用这一特性检测水中痕量的Cd2+。由于Fc-GSH 本身具有氧化还原特性，克服了GSH修饰电极检测金属离子时依靠被测离子的氧化还原特性这一缺点。实验结果表明，用Fc-GSH 修饰电极，对Cd2+的检测极限可达0.1 nM，远远低于GSH 修饰电极 5nM的水平。同时，由于GSH具有很好生物活性的肽，Fc-GSH 这种具有电化学和生物活性的分子，有可能用于生物分子，如蛋白质、酶等的检测。
In this paper, 1’-cysteaminecarbonyl-1-glutathionecarbonyl-ferrocene (Fc-GSH) (4) was firstly synthesized from ferrocene dicarboxylic acid and reduced glutathione (GSH) with several steps. The products were characterized by IR and 1H-NMR. Then Fc-GSH was immobilized on the surface of gold electrode. Cyclic votammetry (CV) was adapted to investigate the electrochemical properties of this Fc-GSH modified electrode in the cadmium ions aqueous solutions. In the presence of cadmium ions,large anodic shift was observed in the experiment. Moreover this shift was proportion to the concentration of Cd2+ when the concentration of cadmium ions is lower than 20 nM. So the modified electrode was used as probes to detect cadmium ions with the limitation of 0.1 nM by cyclic voltammetry.
刘又年、黄可龙、王建秀、曾冬铭、范美意、王芳斌
电化学工业生物化学化学工业概论
二茂铁－谷胱甘肽合成单分子自组装膜修饰电极镉离子检测
ferrocene-glutathione synthesisSAMsmodified electrodecadmium detection
刘又年,黄可龙,王建秀,曾冬铭,范美意,王芳斌.二茂铁基谷胱甘肽修饰电极的制备及其对痕量镉离子的检测[EB/OL].(2006-12-25)[2025-08-11].http://www.paper.edu.cn/releasepaper/content/200612-369.点此复制
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