An in-vitro BBB-on-a-chip open model of human blood-brain barrier enabling advanced optical imaging
An in-vitro BBB-on-a-chip open model of human blood-brain barrier enabling advanced optical imaging
ABSTRACT We describe here the design and implementation of an in-vitro BBB-on-a-chip open model system capable of reconstituting the microenvironment of the blood brain barrier. This system allows controlled unidirectional flow of nutrients and biologicals on the lumen of the artificial microvessel. This BBB-on-a-chip is suitable for high resolution electron microscopy and it is amenable for quantitative 3D live fluorescence imaging using spinning confocal disk or lattice light sheet microscopy (LLSM) to follow, for example the transcytosis across the BBB-like barrier of fluorescently-tagged biological, viruses or nanoparticles.
Ohashi Kazuka G.、de Nola Giovanni、Sano Yasuteru、Salman Mootaz、Marsh Graham、Upadhyayula Srigokul、K¨1sters Ilja、Delinc¨| Matthieu、Di Caprio Giuseppe、Hunt Ronan、Kirchhausen Tom、Shimizu Fumitaka、Kanda Takashi、Obermeier Birgit
Program in Cellular and Molecular Medicine, Boston Children?ˉs HospitalDepartment of Cell Biology, Harvard Medical School||Program in Cellular and Molecular Medicine, Boston Children?ˉs HospitalYamaguchi University Graduate School of MedicineDepartment of Cell Biology, Harvard Medical School||Program in Cellular and Molecular Medicine, Boston Children?ˉs HospitalBiogenDepartment of Cell Biology, Harvard Medical School||Program in Cellular and Molecular Medicine, Boston Children?ˉs Hospital||Department of Molecular and Cell Biology, University of CaliforniaDepartment of Cell Biology, Harvard Medical School||Program in Cellular and Molecular Medicine, Boston Children?ˉs HospitalDepartment of Cell Biology, Harvard Medical School||Program in Cellular and Molecular Medicine, Boston Children?ˉs HospitalDepartment of Cell Biology, Harvard Medical School||Program in Cellular and Molecular Medicine, Boston Children?ˉs HospitalProgram in Cellular and Molecular Medicine, Boston Children?ˉs HospitalDepartment of Cell Biology, Harvard Medical School||Program in Cellular and Molecular Medicine, Boston Children?ˉs Hospital||Department of Pediatrics, Harvard Medical SchoolYamaguchi University Graduate School of MedicineYamaguchi University Graduate School of MedicineBiogen
基础医学生物科学研究方法、生物科学研究技术神经病学、精神病学
blood-brain barriercapillarymicrovesselmicrofluidicslive cell imaging
Ohashi Kazuka G.,de Nola Giovanni,Sano Yasuteru,Salman Mootaz,Marsh Graham,Upadhyayula Srigokul,K¨1sters Ilja,Delinc¨| Matthieu,Di Caprio Giuseppe,Hunt Ronan,Kirchhausen Tom,Shimizu Fumitaka,Kanda Takashi,Obermeier Birgit.An in-vitro BBB-on-a-chip open model of human blood-brain barrier enabling advanced optical imaging[EB/OL].(2025-03-28)[2025-05-21].https://www.biorxiv.org/content/10.1101/2020.06.30.175380.点此复制
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