金属β-内酰胺酶CcrA的热力学研究(II)
etermination of thermokinetic parameters of benzylpenicillin hydrolysis with metallo-β-lactamase CcrA from Bacteroides fragilis
青霉素是临床上使用非常广泛的一种β-内酰胺类抗生素,为了深入研究抗生素耐药问题,本文首次报道了来源于脆弱拟杆菌的金属β-内酰胺酶CcrA催化青霉素水解反应的热力学参数(吉布斯自由能变化值,焓变值,熵变值以及反应活化能)。结果表明该反应在测量温度下为自发放热反应,反应级数为1.4.
In this paper, we report the thermokinetic parameters of benzylpenicillin (as the representative of penicillins) hydrolysis with the B1 subclasses MβL CcrA from Bacteroides fragilis. The activation free energy are 87.90±0.03, 88.99±0.01, 89.93±0.04 and 90.93±0.05 kJomol-1 at 293.15, 298.15, 303.15 and 308.15 K. Activation enthalpy is 29.21±0.03 kJomol-1, activation entropy is -200.34±0.08 Jomol-1oK-1, the reaction order is 1.4, and the apparent activation energy E is 31.71 kJomol-1. The summarized thermokinetic data indicated that CcrA prefer to hydrolyze penicillins among three kinds of β-lactam containing antibiotics.
石莹、翟乐、周立生、杨科武、周亚俊、刘成城
基础医学生物化学药学
细菌耐药微量热法金属β-内酰胺酶 CcrA抗生素水解反应亚胺培南热力学常数
MicrocalorimetryMetallo-β-lactamase CcrAAntibiotic hydrolysisBenzylpenicillinThermokinetic parameters
石莹,翟乐,周立生,杨科武,周亚俊,刘成城.金属β-内酰胺酶CcrA的热力学研究(II)[EB/OL].(2012-07-30)[2025-08-18].http://www.paper.edu.cn/releasepaper/content/201207-314.点此复制
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