胃蛋白酶诱导碳酸钙结晶的研究

alcium salts are often present in the center of all types of gallstones. Matrix proteins are known to be essential for biomineralization and may also be important in the formation and growth of gallstones. In the present work we employed pepsin in order to assess its capability to induce the formation of CaCO3 in biological circumstance in vitro. The appearance and morphology of CaCO3 crystals obtained from systems with and without pepsin were observed by FT-IR, XRD and SEM. The result showed that the pepsin was shown to influence precipitation of CaCO3 from a supersaturated solution in vitro. It suggested that pepsin played an important role in the regulation of precipitation of calcium salts during the formation of gallstones. The result confirmed they were all calcite regardless of the different morphology.