维甲酸诱导腭裂小鼠出生前后Wnt通路相关信号分子的表达
Expression of Signaling Molecules Related to Wnt Pathway in Cleft Palate Induced by Retinoic Acid during Perinatal Stage
目的：Wnt、Shh和BMP等信号分子通路中的重要基因被证实参与到维甲酸致腭裂的过程中。本实验通过验证课题组前期完成的维甲酸诱导腭裂小鼠腭组织全基因组筛选中获得的Wnt信号分子通路相关基因GSK-3β、Fzd3和β-TrCP，从而观察其在正常腭突发育和腭裂形成出生前后表达变化。探讨维甲酸诱导腭裂小鼠出生后对Wnt通路的影响。方法：建立维甲酸诱导小鼠腭裂模型。检测GSK-3β，Fzd3及β-TrCP的mRNA表达水平与蛋白表达部位。结果：1. 出生前后GSK-3β、Fzd3和β-TrCP mRNA表达水平。GSK-3β、Fzd3和β-TrCP 在出生前后、正常腭突及维甲酸诱导腭裂中均具有显著性差异。2. 出生后蛋白分布。WT组中GSK-3β、Fzd3和β-TrCP蛋白表达在腭突上皮，口腔侧腭上皮表达水平较强，在腭间充质中β-TrCP呈弱表达。RA组中GSK-3β、Fzd3和β-TrCP蛋白强表达在腭间充质和腭突上皮，尤其是腭中嵴及口腔侧上皮表达水平更强。结论：出生后维甲酸诱导腭裂小鼠腭上皮中GSK-3β、Fzd3和β-TrCP等Wnt通路抑制因子表达水平上调，提示维甲酸可能通过调控上述基因而对Wnt信号分子通路的活化具有抑制作用。
Objective: Signaling pathways have been shown to participate in the process of Retinoic acid (RA) - induced cleft palate (CP). In our current study, the signaling molecules of GSK-3β, Fzd3 and β-TrCP, which all relate with Wnt pathway, were screened from the palate tissues of the RA-induced CP in mice by the Gene-chip Technology. But, their expression pattern and level in palates during perinatal stages have not been known yet. Method and results: In the studies, mRNA level of GSK-3β, Fzd3 and β-TrCP were detected by quantitative real-time PCR, and showed significant difference between the Embryonic day 18 (ED18) before birth and Postnatal day 0 (PD0) after birth, as well as between the RA-induced CP and wild type during the perinatal stage, respectively. And the localization pattern of GSK-3β, Fzd3 and β-TrCP proteins in palates was also characterized. Conclusions: Our data indicates that the Wnt signaling pathway may involve in the RA-induced cleft palate during peirnatal stage.
吴慧、刘涵、肖晶、丛蔚
基础医学分子生物学生理学
腭裂维甲酸Wnt信号通路动物模型
left palateRetinoic acidWnt SignalsAnimal model
吴慧,刘涵,肖晶,丛蔚.维甲酸诱导腭裂小鼠出生前后Wnt通路相关信号分子的表达[EB/OL].(2013-01-14)[2025-08-02].http://www.paper.edu.cn/releasepaper/content/201301-619.点此复制
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