羧甲基壳聚糖硫酸酯的制备及其对成纤维细胞生长的影响
Synthesis, Characterization of Carboxymethyl Chitosan Sulfate and its Effects on Proliferation of Skin Fibroblasts
采用新型酯化剂三磺酸钠胺(N(SO3Na)3)与羧甲基壳聚糖(CMC)反应，合成了羧甲基壳聚糖硫酸酯(CMCS)。利用红外光谱及核磁共振图谱对产物进行了表征，采用单因素实验法研究了反应的最优条件，同时采用MTT法研究了CMCS对成纤维细胞生长的影响。结果表明，硫酸酯基团含量为26.26%的CMCS在100μg/ml浓度时对成纤维细胞具有促进效果。
arboxymethyl chitosan (CMC) was chemically modified by sulfating agent N(SO3Na)3. The product carboxymethyl chitosan sulfate (CMCS) was characterized by infrared spectroscopy (FT-IR) and 13C NMR. And the optimal reaction conditions were also studied through single factor experiment. MTT method was applied to evaluate the effects of CMCS on proliferation of skin fibroblasts, which demonstrated the concentration of CMCS with sulfate percent of 26.26% at 100 μg/ml promoted the proliferation of skin fibroblasts.
王坦、曹咪、周月、樊李红、高松
基础医学生物科学研究方法、生物科学研究技术
羧甲基壳聚糖硫酸酯三磺酸钠胺成纤维细胞
carboxymethyl chitosan sulfatetrisulfonated sodium amineskin fibroblastsproliferation
王坦,曹咪,周月,樊李红,高松.羧甲基壳聚糖硫酸酯的制备及其对成纤维细胞生长的影响[EB/OL].(2012-02-08)[2025-08-10].http://www.paper.edu.cn/releasepaper/content/201202-153.点此复制
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