过度机械通气早期基因表达变化的实验研究
Expression of Early Response Gene in Lung Due to Injurious Tidal Volume
目的 ： 观察过度通气对早期基因：早期生长因子(Egr－1)、C-jun 和促炎因子IL-1β mRNA、蛋白表达影响及意义。方法： 选择清洁级雄性SD大鼠32只随机分为4组（n=8），戊巴比妥钠腹腔麻醉，小型动物呼吸机给予：VT: 45ml/Kg ， R：40次/min ，I:E：1：2 ； PEEP =0 , 室内空气吸入。于机械通气30、60、90、120min各取肺组织分别以RT-PCR、免疫组化和HE染色方法检测Egr－1、C-jun 、IL-1 mRNA及蛋白表达及肺组织病理改变。结果： 1、机械通气30min时 Egr－1、C-jun 、IL-1βmRNA即有表达，90min达高峰持续至120min，较30min有显著差异（P<0.05）。2、免疫组化：30min时三种蛋白表达不显著，60min时Egr－1、C-jun以胞浆为主，IL-1β略有表达；90－120min，胞浆、胞核染色阳性细胞数增多，IL-1β表达亦增加，较30min时有显著意义（P<0.01）。3、光学显微镜发现：90min肺泡壁略增厚、出血、肺泡腔内渗出和炎症细胞增多，120min加重。结论 ： 机械通气启动和上调早期基因及促炎因子表达，为机械通气肺损伤的早期预测、发病机制研究以及进行基因干预或靶疗提出新的方向。
bjective ：To observe the expression of early response genes due to injurious tidal volume ventilation in adult rat lung in vivo . Methods ： 32 SD male rats were randomly divided into four groups (n=8). The animals were anesthetized by intraperitoneal (ip) injection of 35 mg/kg pentobarbital and placed in dorsal decubitus throughout the experiment.After tracheostomy a 14-gauge cannula was inserted into the trachea. The rats were ventilated at a VT of 45 ml/kg, respiratory rate (RR) of 40 breaths/min, positive end-expiratory pressure (PEEP) of zero cmH2O, and inspired the room air with a ventilator for small animals. Lung tissues were obtained at 30 、60、90、120 min respectively during ventilaition.and then to detect the expression of mRNA of Egr－1, C-jun , IL-1β and the proteins by RT-PCR and immunohistochemical technique ; as well as the Histologic assessment were performed . Results ： At 30min ventilation caused the genes mRNA upregulated of Egr－1、C-jun 、IL-1β , but not with
姚尚龙、武庆平、王月兰、熊平
基础医学
机械通气肺损伤早期基因转录因子
ventilationearly response genetranscription factorpulmonary injury
姚尚龙,武庆平,王月兰,熊平.过度机械通气早期基因表达变化的实验研究[EB/OL].(2005-01-21)[2025-05-02].http://www.paper.edu.cn/releasepaper/content/200501-83.点此复制
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