水牛-猪异种体细胞核移植电融合/激活参数的优化
Optimization of electro-fusion/activation parameters used for buffalo-pig interspecies somatic cell nuclear transfer embryos
异种体细胞核移植是在同种体细胞核移植技术的基础上发展起来的，其在保护濒危物种和器官移植上具有重要的意义。本研究的目的是优化水牛-猪异种体细胞核移植（iSCNT）融合/激活参数，筛选出较优融合/激活条件。首先将iSCNT的重构胚在不同的电融合/激活参数下进行融合/激活，再将iSCNT的重构胚分组进行电融合/激活。结果表明电融合/激活中：用200 V/mm，1次脉冲，30μs的直流脉冲为较优融合/激活参数，并且获得iSCNT囊胚。
Interspecies somatic cell nuclear transfer is of great significance for protecting endangered species. The objective of this study was to optimize the parameters of electric fusion/activation used for activating buffalo-pig interspecies somatic cell nuclear transfer (iSCNT) embryos. Firstly, different electro-activation parameters were used for activating iSCNT embryos. The optimized parameters of one 30-μs DC pulse of 200 V/mm were used for activating iSCNT embryos, and this electro-activation method was sufficient for generating buffalo-pig iSCNT.
崔静辉、卢晟盛
细胞生物学生物工程学遗传学
异种体细胞核移植电融合/激活化学激活
interspecies somatic cell nuclear transferelectro-fusion/activationchemical activation
崔静辉,卢晟盛.水牛-猪异种体细胞核移植电融合/激活参数的优化[EB/OL].(2014-06-16)[2025-08-23].http://www.paper.edu.cn/releasepaper/content/201406-248.点此复制
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