RP-HPLC测定麻黄碱手性对应异构体
Separation and Determination of Chiral Ephedrine and Intermediate with PR-HPLC
用RP-HPLC分离测定了生物转化液中的l-麻黄碱、d-伪麻黄碱及前体物质1-苯基-2-甲胺基丙酮（1-phenyl-2-methylamine-acetone，MAK），采用ZORBAX SB-C18柱，UV检测器，以0.02mol/L的V(KH2PO4):V(甲醇)：V(醋酸)：V(三乙胺)＝96：4：0.1：0.13作为流动相，体积流量1mL/min，柱温35℃，检测波长210nm，MAK、l-麻黄碱、d-伪麻黄碱的检出限分别为0.05，0.2，0.1mg/L，分别测定了两株菌生物转化液中的MAK、l-麻黄碱、d-伪麻黄碱的质量浓度，回收率为97.2%～99.3%。
sensitive and reliable high performance liquid chromatographic method(HPLC)has been developed for the simultaneous determination of l-ephedrine, d-pseudoephedrine and a intermediate 1-phenyl-2-methylamine (MAK) in bioconversion. The HPLC assay was performed on a reversed phase C18 column (ZORBAX SB C18 5 μm 4.6 mm×250 mm) by using methanol-0.2mol/L KH2PO4-acetic acid-triethyl amine (4:96:0.1:0.13,V/V) as mobile phase for analysis. Regression equations revealed the liner relationships (correlation coefficients: 0.9999) between the peak area of each constituent (ephedrine, pseudoephedrine and MAK) and its concentration, The detection limits(S/N=3) for MAK, ephedrine and pseudoephedrine were 0.05mg/L,0.2mg/L and 0.1mg/L, respectively. The bioconversions of the two strains were determined and the recoveries ranged between 97.2%-99.3%.
卢燕、董世建、张梁、石贵阳
药学生物科学研究方法、生物科学研究技术
高效液相色谱法MAKl-麻黄碱d-伪麻黄碱生物转化
HPLC l-ephedrine d-pseudoephedrine MAK biotransformation
卢燕,董世建,张梁,石贵阳.RP-HPLC测定麻黄碱手性对应异构体[EB/OL].(2007-12-14)[2025-08-11].http://www.paper.edu.cn/releasepaper/content/200712-255.点此复制
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