植物表达载体pCAMBIA2300-HaBADH的构建与转化
onstruction of the Plant Expression Vector pCAMBIA2300-HaBADH
本实验采用RT-PCR、RACE等方法从超旱生、耐盐植物梭梭(Haloxylon ammodendron)中扩增出BADH基因的cDNA序列(命名为HaBADH)。根据已获得的开放阅读框，通过双酶切手段将其定向连接入植物表达载体pCAMBIA2300-35s-OCS，构建植物表达载体pCAMBIA2300-HaBADH，并通过农杆菌介导法转化水稻，为进行基因功能验证和通过转基因手段培育耐盐作物奠定了基础。
hrough RT-PCR (reverse transcription polymerase chain reaction) and RACE(rapid amplification of cDNA ends), a betaine aldehyde dehydrogenase (BADH) coding region has been obtained(as named HaBADH) from extreme xerophyte and salt-tolerant plant Haloxylon ammodendron. Use double-digests, construct plant expression vector pCAMBIA2300-HaCMO with obtained fragment and plant expression vector pCAMBIA2300-35s-OCS. Lead the vector pCAMBIA2300-HaCMO into agrobacterium tumefaciens and transform it to rice,, lay a foundation for studying gene function and cultivating salt tolerance crops through transgenosis.
周晓燕、甘晓燕、宋玉霞、石磊
生物工程学遗传学植物学
分子生物学梭梭甜菜碱醛脱氢酶基因克隆转化
Molecular BiologyHaloxylon ammodendronBetaine aldehyde dehydrogenase (BADH)Gene CloningTransform
周晓燕,甘晓燕,宋玉霞,石磊.植物表达载体pCAMBIA2300-HaBADH的构建与转化[EB/OL].(2011-11-23)[2025-08-02].http://www.paper.edu.cn/releasepaper/content/201111-384.点此复制
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