Ex vivo and in vivo imaging of mouse parietal association cortex activity in episodes of cued fear memory formation and retrieval
Ex vivo and in vivo imaging of mouse parietal association cortex activity in episodes of cued fear memory formation and retrieval
Abstract The parietal cortex in rodents has an integrative function and participates in sensory and spatial processing, movement planning and decision-making. However, much less is known about its functions in associative memory processing. Here using Fos immunohistochemical mapping of neuronal activity and two-photon imaging in Fos-eGFP mice we show an involvement of anterior part of the parietal cortex (PtA) in the formation and retrieval of recent fear memory in mice. Using ex vivo c-fos imaging we demonstrate the specific activation of the PtA during recent memory retrieval. In vivo two-photon c-fos imaging confirms these results as well as establishes the activation of the PtA neurons during fear memory formation. Additionally, we describe a design of Fos-Cre-GCaMP transgenic mice to investigate long-term changes of calcium dynamics in neurons captured with Fos-TRAP technique during fear conditioning training.
Anokhin Konstantin V.、Ivashkina Olga I.、Gruzdeva Anna M.、Roshchina Marina A.、Toropova Ksenia A.
Institute for Advanced Brain Studies, Lomonosov Moscow State University||Laboratory for Neurobiology of Memory, P.K. Anokhin Research Institute of Normal PhysiologyLaboratory of Neuroscience, National Research Center ?°Kurchatov Institute?±||Institute for Advanced Brain Studies, Lomonosov Moscow State University||Laboratory for Neurobiology of Memory, P.K. Anokhin Research Institute of Normal PhysiologyLaboratory of Neuroscience, National Research Center ?°Kurchatov Institute?±||Institute for Advanced Brain Studies, Lomonosov Moscow State UniversityCellular Neurobiology of Learning Lab, Institute of Higher Nervous Activity and Neurophysiology of RASLaboratory of Neuroscience, National Research Center ?°Kurchatov Institute?±||Institute for Advanced Brain Studies, Lomonosov Moscow State University||Laboratory for Neurobiology of Memory, P.K. Anokhin Research Institute of Normal Physiology
基础医学神经病学、精神病学生物科学研究方法、生物科学研究技术
parietal association cortexfear memorytransgenic micec-foscalcium activitytwo-photon imaging
Anokhin Konstantin V.,Ivashkina Olga I.,Gruzdeva Anna M.,Roshchina Marina A.,Toropova Ksenia A..Ex vivo and in vivo imaging of mouse parietal association cortex activity in episodes of cued fear memory formation and retrieval[EB/OL].(2025-03-28)[2025-05-05].https://www.biorxiv.org/content/10.1101/863589.点此复制
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