栀子苷与胆酸类成分组合对OGD-Rep模型下SH-SY5Y细胞影响

Objective: acute stroke is the clinical common disease, has the high morbidity and mortality. This study aims to expound bile acids composition and the interaction of geniposide in neural protection effect and mechanism, to adjust JZQKL formula, screening the best combination. Methods: this paper studies the gardenoside and bile acid composition in OGD - Rep model under the influence of SH SY5Y cells, through the determination of CCK 8 cell activity, application of flow cytometry to detect cell apoptosis, and tested the cell supernatant of LDH. Results: low doses of 0.5 mu g/mL HDCA and geniposide pooling, basic did not affect cell activity, and 5, 10 mu g/mL and geniposide share after the cell activity decline; CA is similar to HDCA performance. TUDCA, 5 mu 0.5 g/mL and geniposide share when high cell vitality, GT combination relatively simple Gen slightly higher, there was no statistically significant difference. When TUDCA dose increased, up to 50 ~ 100 mu g/mL, weaken Gen protection of cells, increase the damage in cells. Conclusion: the gardenoside and TUDCA share can play the best nerve protective effect, gardenoside and TUDCA combination preliminary order for those days.